Reporter
GG102

Part:BBa_K2145127

Designed by: Katie Maruna, Laura Chen, Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-19)


GFP/RFP reporter (Reverse/Reverse) with random 500bp spacer

This part contains two fluorescent protein coding sites (RFP and GFP) and was assembled using Golden Gate Assembly. In order to make the plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a 500 base pair "spacer" part derived from different backbones, and a pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 216
    Illegal AgeI site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None